Cultivarium Validates NBx CyClone™ with POSSUM
At Novel Bio, we believe faster, more reliable plasmid DNA production unlocks breakthroughs in genetic medicine. That’s why we’re excited to see independent validation of NBx CyClone™ featured in a recent Cultivarium blog post: POSSUM Unlocks the Biological Universe with Speed.
Breaking Down Barriers with POSSUM
Cultivarium developed POSSUM (Plasmid Origins and Selectable Markers for Undomesticated Microbes), a high-throughput toolkit that quickly determines which plasmids and antibiotic markers function in new microbial hosts. Instead of testing plasmids one at a time, POSSUM screens hundreds of combinations in parallel, compressing what once took months or years into a matter of days.
Why NBx CyClone™ Matters
Among the microbes tested was NBx Cyclone™, Novel Bio’s retail strain of Vibrio natriegens, designed to deliver plasmid DNA up to 3× faster than E. coli. Cultivarium’s team applied POSSUM to NBx CyClone™ without assumptions—treating it like a completely new system—and the results were clear:
Comprehensive compatibility – Identified functional origins of replication and antibiotic markers in under a week.
Aligned with existing knowledge – Results matched Novel Bio’s published guidance and extended it with additional validated options.
Lower switching costs – Scientists gain confidence that their existing plasmids and workflows can translate into Cyclone without months of trial-and-error.
Independent Validation of NBx CyClone™
Cultivarium’s work confirms what many labs are discovering: NBx CyClone™ is more than a fast-growing strain of Vibrio natriegens—it’s a validated platform with low barrier to adoption and accelerates discovery. By combining POSSUM’s high-throughput mapping with Novel Bio’s retail-ready product line, researchers now have data-backed confidence to make the switch from E. coli to a faster, more reliable system for plasmid DNA production.
Looking Ahead
As Cultivarium notes, the implications go beyond one organism. High-throughput approaches like POSSUM can accelerate the adoption of new microbial hosts across biotechnology. For scientists working in gene and cell therapy, CRISPR gene editing, or AAV plasmid production, NBx CyClone™ provides the proven speed and compatibility needed to push research forward.
Read the full Cultivarium blog here or explore NBx CyClone™ on Novel Bio.